Platforms used in miRNA Analysis
By Priyanka Varma
BURLINGAME, Calif.
Dec. 1st, 2022
miRNAs (more than 4500) have been identified and annotated in the miRBase registry [1]. Simple array platform that can detect 553 non-redundant miRNAs encompassing the entire setof miRNAs for human and animal miRNAs have been previously reported [1]. In this article, we will compare the different platforms used in miRNA analysis.
Future research is based on developing lab-on-a-chip (LOC)technology with added advantage of miniaturization and micro-fabrication as well as developing isothermal amplification-based assays and lateral-flow assay based systems [3].
Bibliography:
1. Tang X, Gal J, Zhuang X, Wang W, Zhu H, Tang G.A simple array platform for microRNA analysis and its application in mouse tissues. RNA. 2007 Oct;13(10):1803-22. doi: 10.1261/rna.498607. Epub 2007 Aug3. PMID: 17675362; PMCID: PMC1986807.
2. McClure LV, Lin YT, Sullivan CS. Detection of viral microRNAs by Northern blot analysis. Methods Mol Biol. 2011;721:153-71.doi: 10.1007/978-1-61779-037-9_9. PMID: 21431684.
3. Dave, V.P., Ngo, T.A., Pernestig, AK. et al. MicroRNA amplification and detection technologies: opportunities and challenges for point of care diagnostics. Lab Invest 99, 452–469 (2019).https://doi.org/10.1038/s41374-018-0143-3
4. Krichevsky AM, King KS, Donahue CP, Khrapko K, Kosik KS. A microRNA array reveals extensive regulation of microRNAs during brain development. RNA. 2003 Oct;9(10):1274-81. doi: 10.1261/rna.5980303.Erratum in: RNA. 2004 Mar;10(3):551. PMID: 13130141; PMCID: PMC1370491.
5. miRNAAnalysis | Thermo Fisher Scientific - US
7. TaqMan Advanced miRNA Assays | Thermo Fisher Scientific - US
8. Gardner SN, Kuczmarski TA, Vitalis EA, Slezak TR. Limitations of TaqMan PCR for detecting divergent viral pathogensillustrated by hepatitis A, B, C, and E viruses and human immunodeficiencyvirus. J Clin Microbiol. 2003 Jun;41(6):2417-27. doi:10.1128/JCM.41.6.2417-2427.2003. PMID: 12791858; PMCID: PMC156483.
9. Pharmacogenomics:Methodologies for genotyping and phenotyping - ScienceDirect
10. QuantiGenemiRNA Singleplex Assay | Thermo Fisher Scientific - US
11. Jin F, Xu D. A Cascaded DNA Circuit in BeadArrays for Quantitative Single-Cell MicroRNA Analysis. Anal Chem. 2021 Aug24;93(33):11617-11625. doi: 10.1021/acs.analchem.1c02388. Epub 2021 Aug 10.PMID: 34375096.
